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To collect the large quantities of embryos necessary to build a gene expression atlas, we used the maternal Gal4 shRNA system to deplete bcd mRNA in the female germ line Ni et al, 2011, Staller et al, 2013 . Globally, our analyses revealed a relatively modest impact of lid KD and Sin3A KD on ovarian gene expression, with more genes downregulated in both cases Fig 2B and S5 Fig, S2 Table . To clarify the effects of srl overexpression on the phenotype of tko 25t we conducted a number of tests in which we varied the overexpression construct used and the genetic background. Our tests ordered these maternal drivers into the relatively weak nos-Gal4, the moderate MTD-Gal4 driver, and the strong mat-Gal4-2+3 driver carrying maternal -tubulin-GAL4 drivers on both the second and third chromosomes shRNA and GAL4 lines used are described in detail in Table 2 in Fly stocks later in this article .
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Specifications are provided by the manufacturer. Shop and buy Smart Array Controllers and Smart Host Bus Adapters from Hewlett Packard Enterprise, view our categories, sort filter and refine to narrow your selection.
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